Iron plays a vital role in several important processes in our body, including transporting oxygen, helping our cells produce energy, supporting DNA synthesis, activating immune cells, and maintaining balance within our cells. Moreover, iron is also involved in regulating inflammation and aiding in the body's repair processes. This makes it a strong candidate for enhancing the resilience and longevity of our cells.
As interest in iron-based supplements grows, especially in relation to enhancing immune health, metabolic function, and promoting healthy aging, this study explored whether Synthesit Iron can stimulate positive cellular responses without causing harmful or inflammatory effects. By utilizing a targeted gene expression analysis of primary human immune cells, the study aimed to provide solid preclinical evidence supporting the health benefits associated with Synthesit Iron.
For this reason, the research team of Synthesit decided to conduct a further scientific analysis. In June 2025 we received further proof of the powerful effects of Synthesit Iron. The main goal of this in-vitro study was to scientifically assess the effects of Synthesit Iron (Iron(III) Citrate) on human immune cells, particularly looking at how it influences macrophage function, gene expression, and the regulation of metabolism related to the immune system.
This research aimed to provide insights into how Synthesit Iron affects crucial processes in cells, including:
- inflammation control,
- response to oxidative stress,
- energy metabolism,
- mitochondrial health,
- signaling related to longevity, and
- regulation of genes linked to diabetes.
The analysis used a focused panel of 100 relevant genes to explore the effects of Synthesit Iron. After incubating with Iron(III) Citrate for 24 hours, primary human immune cells showed a consistent and meaningful increase in important genes related to protecting against oxidative stress, maintaining metabolic balance, and promoting cellular longevity. Notably, all changes in gene expression were within a physiologically relevant range, showing an increase of up to 100%. This indicates that the cells are adapting positively to low doses of non-toxic iron exposure.
Anti-inflammatory effect:
The analysis looked at genes that counteract inflammation, promote immune resolution, and facilitate tissue repair. Usage of Synthesit Iron showed an elevated expression of these genes and suggests wound healing, immunoregulation, and anti-inflammatory signaling. These changes imply that Synthesit Iron can downregulate harmful immune overactivation while fostering a restorative environment.
The expression changes observed range from 15% to 85%, representing a consistent and biologically meaningful activation of anti-inflammatory pathways without indicating toxic responses.
For example, Interleukin-10 (IL-10) is a hallmark anti-inflammatory cytokine that showed an upregulation of approximately 85%.
Effect on metabolism:
The analysis looked at genes involved in mitochondrial activity, energy production, and metabolic reprogramming. Key markers indicated an increase in activity, suggesting a shift towards oxidative phosphorylation and improved mitochondrial efficiency. This metabolic change helps maintain immune cell function and lowers the risk of metabolic burnout in chronic conditions.
The observed upregulation — ranging from 23% to 100% — indicates a broad metabolic activation consistent with enhanced mitochondrial capacity and stress-adaptive responses, without triggering cytotoxic stress.
For example, PGC1A (74% upregulation) enhances mitochondrial renewal and energy output—crucial for cellular endurance, immune cell function, and aging resilience.
Anti-diabetic effect:
The diabetes related genes help assess the anti-diabetic and insulin sensitizing potential of Synthesit Iron. Results showed modulation of key regulators that reduce insulin resistance and promote glucose utilization, suggesting that Synthesit Iron may positively influence metabolic inflammation.
The majority of genes demonstrated positive regulation, while TNF was downregulated, indicating a shift toward an anti-diabetic and insulin sensitizing gene expression profile.
For example, Tumor Necrosis Factor Alpha (TNF-α) is a pro-inflammatory cytokine that impairs insulin signaling and contributes to metabolic dysfunction. Its significant downregulation of 23% is a strong indicator of anti-inflammatory activity and potential insulin-sensitizing effects of Iron(III) Citrate.
Effect on longevity:
The study looked at genes connected to cellular lifespan extension, stress resilience, and repair mechanisms. An upregulation of these multiple pro-longevity markers reflect a robust activation of antioxidant pathways and longevity-related transcription factors. These responses indicate that Synthesit Iron not only mitigates oxidative damage but may also enhance cellular defenses associated with healthy aging and immune balance.
For example, Forkhead Box O3 (FOXO3) is a longevity-associated gene involved in DNA repair, autophagy, and oxidative stress response. An upregulation of FOXO3 by 74% due to Synthesit Iron indicates improved cellular resilience, immune balance, and reduced inflammatory signaling — hallmarks of healthy aging.
For a more detailed understanding please have a look at the full report here:
The results show that Synthesit Iron can activate a wide range of beneficial genes related to protection, healing, and metabolism. This suggests that it could be a valuable food supplement for various areas, including aging, boosting the immune system, helping with energy production, and aiding in detoxification of the body.
Synthesit Iron is especially important in the context of chronic inflammation and offers exciting possibilities for enhancing longevity and healthy aging
